10.46243/jst.2021.v6.i6.pp19-30 registered
The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes.
Resolves to https://www.jst.org.in/index.php/pub/article/view/517
Held by Longman Publishers (India) · prefix 10.46243 live · DOI address https://doi.org/10.46243/jst.2021.v6.i6.pp19-30
Registered 29 Sep 2026 via crossref · record version 2 · last change 29 Sep 2026, 11:59 PM · record sha256 7e8d357ac0066fa4…
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JournalArticle — an article in a journal · Digital · Visual · en
The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes. (PrincipalTitle)
Published 2021-12-10
Part of Journal of Science & Technology · ISSN 2456-5660 · volume 06 · issue 06 · pages 19–30
Agents
- Dr.A Padmanabham (author)
- Mr.V.N.V.Radha Kishna Murty (author)
- Longman Publishers (publisher)
Identifiers DOI 10.46243/jst.2021.v6.i6.pp19-30
Abstract
Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in biomedical materials and implant coatings; however, their effects on osteoblast function may vary according to particle size, morphology, crystalline phase, concentration, and exposure duration. This study evaluated the cytotoxicity and osteogenic effects of four uncoated TiO₂ nanoparticles—two anatase particles (A1 and A2) with erythrocyte-like and spherical morphologies and two rod-shaped rutile particles (R1 and R2)—using MC3T3-E1 mouse preosteoblast cells. Particle morphology, size, surface characteristics, dispersion, and aggregation were examined using scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, dynamic light scattering, and zeta-potential analysis. Cell viability was measured using the CCK-8 assay, while apoptosis, reactive oxygen species generation, antioxidant status, alkaline phosphatase activity, osteocalcin production, cellular ultrastructure, and mineralized-nodule formation were also assessed. TiO₂ nanoparticles exhibited concentration-, size-, shape-, and exposure-dependent biological effects. Concentrations above 20 μg/mL adversely affected cell viability, with reductions exceeding 50% at concentrations above 50 μg/mL. Exposure to the nanoparticles decreased alkaline phosphatase activity and inhibited osteogenic differentiation, although osteocalcin levels did not differ significantly from the control. TiO₂ treatment also increased reactive oxygen species and superoxide production, altered antioxidant parameters, and produced cellular and organelle damage following nanoparticle internalization. These findings indicate that TiO₂ nanoparticles may negatively affect preosteoblast proliferation and differentiation through oxidative stress and intracellular structural damage. Careful optimization of nanoparticle physicochemical characteristics and exposure levels is therefore necessary when incorporating TiO₂ nanoparticles into materials intended for bone-related biomedical applications.
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Each element by the standard's name (Annex B: reference elements, then administrative) and the Handbook's (in grey), read off the record above.
| Element | Value | In the record |
|---|---|---|
| DOI Name DOI name | 10.46243/jst.2021.v6.i6.pp19-30 | doi |
| Referent Type referentType | Creation | referent |
| Referent Sub-Type referentSubType | JournalArticle — an article in a journal | type |
| Referent Name(s) referentName(s) | The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes. (PrincipalTitle, en) | titles |
| Basic Metadata basicMetadata | author: Dr.A Padmanabham author: Mr.V.N.V.Radha Kishna Murty publisher: Longman Publishers published: 2021-12-10 part of: Journal of Science & Technology · ISSN 2456-5660 · vol. 06 · no. 06 · pp. 19–30 language: en form: Digital · Visual · Language | agents, dates, container, language, structural_type, modes, characters |
| Referent Identifier(s) alternateIdentifier(s) | none besides the DOI | identifiers, relations (IsSameAs) |
| Registration Authority registrationAuthorityCode | Crossref — issued by Crossref (member 25296); held here as a copy | record.source_agency (our code, ra_doi_name, for names issued here once appointed) |
| Created Date issueDate | 2026-09-08 | record.registered (when the DOI name was first registered) |
| relatedIdentifiers | none needed — the descriptive metadata is in this record | container, relations (only where the descriptive metadata lives at another identifier) |
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Every change to this DOI, in order, as it was recorded. Entries are only ever added, never changed or removed.
| # | When | What | By | Changes |
|---|---|---|---|---|
| 1 | 29 Sep 2026, 10:00 PM | register registered at Crossref; record read from api.crossref.org | Administrator (admin) | 115 fields set · sha256 a3f931dfc368… |
| 2 | 29 Sep 2026, 11:59 PM | update record re-read from api.crossref.org | Administrator (admin) | container.titles.0.value: |
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