Cite this DOI

Padmanabham, D., & Kishna Murty, M. (2021). The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes. *Journal of Science & Technology*, *06*(06), 19–30. https://doi.org/10.46243/jst.2021.v6.i6.pp19-30

@article{padmanabham2021osteogenic,
  author    = {Padmanabham, Dr.A and Kishna Murty, Mr.V.N.V.Radha},
  title     = {{The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes.}},
  journal   = {Journal of Science \& Technology},
  year      = {2021},
  month     = {dec},
  volume    = {06},
  number    = {06},
  pages     = {19--30},
  publisher = {Longman Publishers},
  issn      = {2456-5660},
  doi       = {10.46243/jst.2021.v6.i6.pp19-30},
  url       = {https://doi.org/10.46243/jst.2021.v6.i6.pp19-30},
  language  = {en},
  abstract  = {Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in biomedical materials and implant coatings; however, their effects on osteoblast function may vary according to particle size, morphology, crystalline phase, concentration, and exposure duration. This study evaluated the cytotoxicity and osteogenic effects of four uncoated TiO₂ nanoparticles—two anatase particles (A1 and A2) with erythrocyte-like and spherical morphologies and two rod-shaped rutile particles (R1 and R2)—using MC3T3-E1 mouse preosteoblast cells. Particle morphology, size, surface characteristics, dispersion, and aggregation were examined using scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, dynamic light scattering, and zeta-potential analysis. Cell viability was measured using the CCK-8 assay, while apoptosis, reactive oxygen species generation, antioxidant status, alkaline phosphatase activity, osteocalcin production, cellular ultrastructure, and mineralized-nodule formation were also assessed. TiO₂ nanoparticles exhibited concentration-, size-, shape-, and exposure-dependent biological effects. Concentrations above 20 μg/mL adversely affected cell viability, with reductions exceeding 50\% at concentrations above 50 μg/mL. Exposure to the nanoparticles decreased alkaline phosphatase activity and inhibited osteogenic differentiation, although osteocalcin levels did not differ significantly from the control. TiO₂ treatment also increased reactive oxygen species and superoxide production, altered antioxidant parameters, and produced cellular and organelle damage following nanoparticle internalization. These findings indicate that TiO₂ nanoparticles may negatively affect preosteoblast proliferation and differentiation through oxidative stress and intracellular structural damage. Careful optimization of nanoparticle physicochemical characteristics and exposure levels is therefore necessary when incorporating TiO₂ nanoparticles into materials intended for bone-related biomedical applications.}
}

TY  - JOUR
TI  - The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes.
AU  - Padmanabham, Dr.A
AU  - Kishna Murty, Mr.V.N.V.Radha
JO  - Journal of Science & Technology
PY  - 2021
DA  - 2021/12/10/
VL  - 06
IS  - 06
SP  - 19
EP  - 30
PB  - Longman Publishers
SN  - 2456-5660
LA  - en
AB  - Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in biomedical materials and implant coatings; however, their effects on osteoblast function may vary according to particle size, morphology, crystalline phase, concentration, and exposure duration. This study evaluated the cytotoxicity and osteogenic effects of four uncoated TiO₂ nanoparticles—two anatase particles (A1 and A2) with erythrocyte-like and spherical morphologies and two rod-shaped rutile particles (R1 and R2)—using MC3T3-E1 mouse preosteoblast cells. Particle morphology, size, surface characteristics, dispersion, and aggregation were examined using scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, dynamic light scattering, and zeta-potential analysis. Cell viability was measured using the CCK-8 assay, while apoptosis, reactive oxygen species generation, antioxidant status, alkaline phosphatase activity, osteocalcin production, cellular ultrastructure, and mineralized-nodule formation were also assessed. TiO₂ nanoparticles exhibited concentration-, size-, shape-, and exposure-dependent biological effects. Concentrations above 20 μg/mL adversely affected cell viability, with reductions exceeding 50% at concentrations above 50 μg/mL. Exposure to the nanoparticles decreased alkaline phosphatase activity and inhibited osteogenic differentiation, although osteocalcin levels did not differ significantly from the control. TiO₂ treatment also increased reactive oxygen species and superoxide production, altered antioxidant parameters, and produced cellular and organelle damage following nanoparticle internalization. These findings indicate that TiO₂ nanoparticles may negatively affect preosteoblast proliferation and differentiation through oxidative stress and intracellular structural damage. Careful optimization of nanoparticle physicochemical characteristics and exposure levels is therefore necessary when incorporating TiO₂ nanoparticles into materials intended for bone-related biomedical applications.
DO  - 10.46243/jst.2021.v6.i6.pp19-30
UR  - https://doi.org/10.46243/jst.2021.v6.i6.pp19-30
ER  -

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    "DOI": "10.46243/jst.2021.v6.i6.pp19-30",
    "URL": "https://doi.org/10.46243/jst.2021.v6.i6.pp19-30",
    "title": "The Osteogenic Potential of Titanium Dioxide Nanoparticles of Different Sizes and Shapes.",
    "source": "Smart Scholars DOI Registry",
    "container-title": "Journal of Science & Technology",
    "author": [
        {
            "family": "Padmanabham",
            "given": "Dr.A"
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            "family": "Kishna Murty",
            "given": "Mr.V.N.V.Radha"
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            [
                2021,
                12,
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    "volume": "06",
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    "publisher": "Longman Publishers",
    "language": "en",
    "abstract": "Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in biomedical materials and implant coatings; however, their effects on osteoblast function may vary according to particle size, morphology, crystalline phase, concentration, and exposure duration. This study evaluated the cytotoxicity and osteogenic effects of four uncoated TiO₂ nanoparticles—two anatase particles (A1 and A2) with erythrocyte-like and spherical morphologies and two rod-shaped rutile particles (R1 and R2)—using MC3T3-E1 mouse preosteoblast cells. Particle morphology, size, surface characteristics, dispersion, and aggregation were examined using scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, dynamic light scattering, and zeta-potential analysis. Cell viability was measured using the CCK-8 assay, while apoptosis, reactive oxygen species generation, antioxidant status, alkaline phosphatase activity, osteocalcin production, cellular ultrastructure, and mineralized-nodule formation were also assessed. TiO₂ nanoparticles exhibited concentration-, size-, shape-, and exposure-dependent biological effects. Concentrations above 20 μg/mL adversely affected cell viability, with reductions exceeding 50% at concentrations above 50 μg/mL. Exposure to the nanoparticles decreased alkaline phosphatase activity and inhibited osteogenic differentiation, although osteocalcin levels did not differ significantly from the control. TiO₂ treatment also increased reactive oxygen species and superoxide production, altered antioxidant parameters, and produced cellular and organelle damage following nanoparticle internalization. These findings indicate that TiO₂ nanoparticles may negatively affect preosteoblast proliferation and differentiation through oxidative stress and intracellular structural damage. Careful optimization of nanoparticle physicochemical characteristics and exposure levels is therefore necessary when incorporating TiO₂ nanoparticles into materials intended for bone-related biomedical applications.",
    "ISSN": "2456-5660"
}

From the record as registered (version 2) — the record and its history. Programs: https://registry.smartscholars.in/api.php?action=cite&doi=10.46243%2Fjst.2021.v6.i6.pp19-30 gives all four in one JSON answer.