{ "ok": true, "doi": "10.46243/jstj.2020.v5.i2.302", "doi_display": "10.46243/jstj.2020.v5.i2.302", "doi_url": "https://doi.org/10.46243/jstj.2020.v5.i2.302", "state": "registered", "url": "https://www.jst.org.in/index.php/pub/article/view/296", "title": "A Review on Gene Expression In Trypanosomes", "version": 2, "registered_via": "crossref", "prefix": { "prefix": "10.46243", "status": "live" }, "registrant": { "name": "Longman Publishers", "kind": "publisher", "country": "India" }, "reserved_at": null, "registered_at": "2026-09-29 22:00:37", "updated_at": "2026-09-29 23:59:57", "withdrawn_at": null, "withdrawn_reason": null, "record": { "format": "smartscholars-doi-metadata/1.0", "doi": "10.46243/jstj.2020.v5.i2.302", "referent": "Creation", "type": "JournalArticle", "structural_type": "Digital", "modes": [ "Visual" ], "characters": [ "Language" ], "titles": [ { "value": "A Review on Gene Expression In Trypanosomes", "type": "PrincipalTitle", "lang": "en" } ], "identifiers": [ { "type": "DOI", "value": "10.46243/jstj.2020.v5.i2.302" } ], "agents": [ { "role": "author", "name": { "given": "Shafinaz", "family": "Filwa S" }, "sequence": "first", "affiliations": [ { "name": "Department of Biomedical Engineering, Saveetha School of Engineering, Thandalam, Chennai, Tamilnadu, India" } ] }, { "role": "author", "name": { "given": "Nanmaran", "family": "R" }, "sequence": "additional", "affiliations": [ { "name": "Department of Biomedical Engineering, Saveetha School of Engineering, Thandalam, Chennai, Tamilnadu, India" } ] }, { "role": "publisher", "name": { "org": "Longman Publishers" } } ], "dates": { "published": "2020", "date_type": "PublicationDate", "online": "2020" }, "language": "en", "container": { "type": "Journal", "titles": [ { "value": "Journal of Science & Technology", "type": "PrincipalTitle" } ], "identifiers": [ { "type": "ISSN", "value": "2456-5660", "medium": "electronic" } ], "volume": "05", "issue": "02", "pages": { "first": "110", "last": "112" } }, "links": [ { "url": "https://www.jst.org.in/index.php/pub/article/view/296", "return_type": "text/html", "primary": true }, { "url": "https://www.jst.org.in/index.php/pub/article/download/296/258", "purpose": "text-mining", "return_type": "application/pdf" }, { "url": "https://www.jst.org.in/index.php/pub/article/download/296/1322", "purpose": "text-mining", "return_type": "application/xml" } ], "abstract": { "value": "Unique To progress transfection productivity in Trypanosomacruzi, we created a modern electroporation protocol and expression vectors which utilize luciferase and green and ruddy fluorescent proteins as columnist qualities. In transients transfections, the electroporation conditions reported here brought about in luciferase expression 100 times higher than the levels gotten with already described protocols. To confirm whether groupings containing different trans-splicing signals impact columnist gene expression, we compared DNA parts corresponding to 5¢ untranslated furthermore intergenic (5¢ UTR furthermore Ig) regions from GAPDH, TcP2b, a-and b-tubulin and amastin qualities. Vectors containing groupings derived from the primary four qualities displayed comparative efficiencies and brought about in luciferase expression in transiently transfected epimastigotes that was up to 10 timeshigher than that for a control vector. In differentiate, the amastin 5¢ UTR also Ig come about in lower levels of columnist gene expressionWe too developed a vector containing an expression cassette planned to be focused on to the tubulin locus of the parasite[1].", "lang": "en" }, "license": { "url": "https://creativecommons.org/licenses/by/4.0/", "start": "2020-01-01", "applies_to": "vor" }, "references": [ { "key": "ref1", "doi": "10.1073/pnas.1600304113", "unstructured": "Aksoy E., Vigneron A., Binga X. L., Zhaoa X., O'Neill M., Wua Y., et al. (2016). Mammalian African trypanosome VSG coat improves" }, { "key": "ref2", "unstructured": "tsetse's vector ability. Proc. Natl. Acad. Sci. U.S.A. 113 6961–6966" }, { "key": "ref3", "doi": "10.1016/s0140-6736(03)14694-6", "unstructured": "Barrett M. P., Burchmore R. J., Stich A., Lazzari J. O., Frasch A. C., Cazzulo J. J., et al. (2003). The trypanosomiases. Lancet 362 1469–1480" }, { "key": "ref4", "unstructured": "Barry J. D., Emergy D. L. (1984). 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Human host determinants impacting the result" }, { "key": "ref17", "unstructured": "of Trypanosoma brucei gambiense contaminations. Parasite Immunol" } ], "record": { "registrant": "Longman Publishers", "registered": "2026-09-08", "updated": "2026-09-28", "issue_number": 1, "source": "crossref-api", "source_agency": "Crossref (member 25296)" } }, "record_sha256": "9c5d4332a370937e41b66912deb8348e1d732fe1703cc6f6a6ad0e71cf327d6a", "handle": { "synced_at": "2026-10-01 18:13:41", "url": "https://www.jst.org.in/index.php/pub/article/view/296" }, "links": { "record_page": "https://registry.smartscholars.in/record.php?doi=10.46243%2Fjstj.2020.v5.i2.302", "system_metadata": "https://registry.smartscholars.in/resolve.php?doi=10.46243%2Fjstj.2020.v5.i2.302&as=system", "history": "https://registry.smartscholars.in/api.php?action=history&doi=10.46243%2Fjstj.2020.v5.i2.302", "kernel_xml": "https://registry.smartscholars.in/resolve.php?doi=10.46243%2Fjstj.2020.v5.i2.302&as=xml" } }