{ "ok": true, "doi": "10.46243/jst.2022.v7.i01.pp49-61", "events": [ { "seq": 1, "kind": "register", "at": "2026-09-29 22:00:23", "by": "Administrator (admin)", "by_kind": "user", "note": "registered at Crossref; record read from api.crossref.org", "changes": [ { "path": "abstract.lang", "from": null, "to": "en" }, { "path": "abstract.value", "from": null, "to": "Methicillin-resistant Staphylococcus aureus (MRSA) is a human pathogen that has emerged as a serious public health problem both in hospital and community settings. This study was aimed to determine the clonal variations of MRSA from healthcare and community environments. S. aureus was isolated and identified from hospital and community samples using standard laboratory procedures and MRSA strains were identified using Kirby Bauer disk diffusion method. Out of 505 S. aureus isolates recovered in this study, 295 (58.4%) were oxacillin resistant and were therefore considered to be methicillin-resistant Staphylococcus aureus (MRSA) strains. The prevalence of MRSA in the hospital environment was 34.8% and 28.7% in the community environment. Out of 21 selected MRSA isolates examined for mecA gene by PCR, only six (53, 69, 74, 84, 88 and 89) showed positive amplification for mecA. Of these six, one was a hospital isolate, while the other five were community isolates. Polymerase chain reaction (PCR) was used to detect both the Staphylococcus specific 16s rRNA and mecA genes with amplicon sizes of 750 bp and 310 bp, All oxacillin resistant isolates possessed the S. aureus specific sequence gene, but not all possessed the mecA gene. Genetic relatedness of the isolates was determined using protein profile from SDS-PAGE and restriction patterns from restriction fragment length polymorphism (RFLP). The protein profiles and restriction patterns revealed heterogeneity among the isolates. Phylogenetic analyses were done on the protein profiles and restriction patterns using bioinformatics software (UPGMA). 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Journal of Clinical Microbiology. 2004; 42:4947-4955" }, { "path": "references.16.key", "from": null, "to": "ref17" }, { "path": "references.16.unstructured", "from": null, "to": "Harlow ED, Lane D. Antibodies: Laboratory manual.CSHL press. 1988; Pp 484–493" }, { "path": "references.17.doi", "from": null, "to": "10.1203/pdr.0b013e31819dc44d" }, { "path": "references.17.key", "from": null, "to": "ref18" }, { "path": "references.17.unstructured", "from": null, "to": "Liu GY. Molecular pathogenesis of Staphylococcus aureus infection. Pediatric Research. 2009; 65:71R-77R" }, { "path": "references.18.doi", "from": null, "to": "10.9734/jamb/2017/33655" }, { "path": "references.18.key", "from": null, "to": "ref19" }, { "path": "references.18.unstructured", "from": null, "to": "Fagade OE, Ezeamagu CO, Oyelade AA, Ogunjobi AA. Comparative study of antibiotic resistance of Staphylococcus species isolated from clinical and environmental samples. Assumption University Journal of Technology. 2010; 13: 165-169. List of Reviewer Professor Christopher Anyamene Department of Applied Microbiology and Brewing Nnamdi Azikiwe University, Awka Anambra State, Nigeria c.anyamene@unizik.edu.ng" }, { "path": "references.2.doi", "from": null, "to": "10.1016/j.ajic.2007.10.009" }, { "path": "references.2.key", "from": null, "to": "ref3" }, { "path": "references.2.unstructured", "from": null, "to": "Jarvis WR, Schlosser J, Chinn RY, Tweeten S, Jackson M. National prevalence of methicillin-resistant Staphylococcus aureus in inpatients at US health care facilities. American Journal of Infection Control.2007; 35: 631-637" }, { "path": "references.3.doi", "from": null, "to": "10.3201/eid0806.010313" }, { "path": "references.3.key", "from": null, "to": "ref4" }, { "path": "references.3.unstructured", "from": null, "to": "Salmenlinna S, Lyytikainen O, Vuopio-Varkila J. Community-acquired methicillin-resistant Staphylococcus aureus, Finland. Emerging Infectious Diseases.2002; 8: 602-607" }, { "path": "references.4.doi", "from": null, "to": "10.1016/j.ijid.2007.03.004" }, { "path": "references.4.key", "from": null, "to": "ref5" }, { "path": "references.4.unstructured", "from": null, "to": "Akpaka PE, Kissoon S, Rutherford C, Swanston WH, Jayaratne P. Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from regional hospitals in Trinidad and Tobago. International Journal of Infectious Diseases.2007; 11: 544–548" }, { "path": "references.5.key", "from": null, "to": "ref6" }, { "path": "references.5.unstructured", "from": null, "to": "Al-Ruaily MA, Khahl OM. Detection of (mecA) gene in methicillin resistance Staphylococcus aureus (MRSA) at prince A/Rhman Sidery hospital, Al-Jouf, Saudi Arabia. Journal of Medical Genetics and Genomics.2011; 3(3): 41–45" }, { "path": "references.6.doi", "from": null, "to": "10.1128/jcm.00044-07" }, { "path": "references.6.key", "from": null, "to": "ref7" }, { "path": "references.6.unstructured", "from": null, "to": "Popovich K, Hota B, Rice T, Aroutcheva A, Weinstein RA. Phenotypic prediction rule for community-associated methicillin- resistant Staphylococcus aureus. Journal of Clinical Microbiology.2007; 45: 2293-2295" }, { "path": "references.7.doi", "from": null, "to": "10.1371/journal.ppat.1003134" }, { "path": "references.7.key", "from": null, "to": "ref8" }, { "path": "references.7.unstructured", "from": null, "to": "Kouyos R, Klein E, Grenfell, B. Hospital-community interactions foster coexistence between methicillin- resistant strains of Staphylococcus aureus. 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This study was aimed to determine the clonal variations of MRSA from healthcare and community environments. S. aureus was isolated and identified from hospital and community samples using standard laboratory procedures and MRSA strains were identified using Kirby Bauer disk diffusion method. Out of 505 S. aureus isolates recovered in this study, 295 (58.4%) were oxacillin resistant and were therefore considered to be methicillin-resistant Staphylococcus aureus (MRSA) strains. The prevalence of MRSA in the hospital environment was 34.8% and 28.7% in the community environment. Out of 21 selected MRSA isolates examined for mecA gene by PCR, only six (53, 69, 74, 84, 88 and 89) showed positive amplification for mecA. Of these six, one was a hospital isolate, while the other five were community isolates. Polymerase chain reaction (PCR) was used to detect both the Staphylococcus specific 16s rRNA and mecA genes with amplicon sizes of 750 bp and 310 bp, All oxacillin resistant isolates possessed the S. aureus specific sequence gene, but not all possessed the mecA gene. Genetic relatedness of the isolates was determined using protein profile from SDS-PAGE and restriction patterns from restriction fragment length polymorphism (RFLP). The protein profiles and restriction patterns revealed heterogeneity among the isolates. Phylogenetic analyses were done on the protein profiles and restriction patterns using bioinformatics software (UPGMA). The results revealed four different patterns suggesting that there are about four MRSA clones among the samples as well as genetic relatedness between hospital and community isolates.", "lang": "en" }, "license": { "url": "https://creativecommons.org/licenses/by/4.0", "start": "2023-07-26", "applies_to": "vor" }, "references": [ { "key": "ref1", "unstructured": "Chatterjee SS, Ray P, Aggarwal A, Das A, Sharma M. A community-based study on nasal carriage of Staphylococcus aureus. Indian Journal of Medical Research. 2009; 130: 742-748" }, { "key": "ref2", "doi": "10.1038/srep01902", "unstructured": "Prosperi M, Veras N, Azarian T, Rathore M.,Nolan D, Rand K, Cook RL, Johnson J, Morris JG, Salemi M. Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in the genomic era: a cross-sectional study. Scientic Reports3.2013" }, { "key": "ref3", "doi": "10.1016/j.ajic.2007.10.009", "unstructured": "Jarvis WR, Schlosser J, Chinn RY, Tweeten S, Jackson M. National prevalence of methicillin-resistant Staphylococcus aureus in inpatients at US health care facilities. American Journal of Infection Control.2007; 35: 631-637" }, { "key": "ref4", "doi": "10.3201/eid0806.010313", "unstructured": "Salmenlinna S, Lyytikainen O, Vuopio-Varkila J. Community-acquired methicillin-resistant Staphylococcus aureus, Finland. Emerging Infectious Diseases.2002; 8: 602-607" }, { "key": "ref5", "doi": "10.1016/j.ijid.2007.03.004", "unstructured": "Akpaka PE, Kissoon S, Rutherford C, Swanston WH, Jayaratne P. Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from regional hospitals in Trinidad and Tobago. International Journal of Infectious Diseases.2007; 11: 544–548" }, { "key": "ref6", "unstructured": "Al-Ruaily MA, Khahl OM. Detection of (mecA) gene in methicillin resistance Staphylococcus aureus (MRSA) at prince A/Rhman Sidery hospital, Al-Jouf, Saudi Arabia. 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Journal of Clinical Microbiology.2005; 43: 421-426" }, { "key": "ref10", "doi": "10.1371/journal.pone.0151937", "unstructured": "Ilczyszyn WM, Sabat AJ, Akkerboom V, Szkariat A, Klepacka J, Sowa-Sierant I, Wasik, B, Kosecka-Strojek M, Buda A, Miedzobrodzki J,Friedrich AW. Clonal structure and characterization of Staphylococcus aureus strains from invasive infections in paediatric patients from South Poland: Association between Age, spa types, clonal complexes and genetic markers. PloS ONE.2016; 11(3): e0151937" }, { "key": "ref11", "doi": "10.1016/j.micpath.2017.01.055", "unstructured": "Goudarzi M, Seyedjavadi SS, Nasiri MJ, Goudarzi H, Nia RS, Dabiri H. Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa and agr locus types analysis. Microbiology and Pathogen.2017; 104: 328-335" }, { "key": "ref12", "doi": "10.1371/journal.pone.0247013", "unstructured": "Akinduti AP, Osiyemi J A, Banjot T, Ejilude O, El-Ashker M, Adeyemi AG, Obafemi Y D, Isibor P O. Clonal diversity and spatial dissemination of multi-antibiotics resistant Staphylococcus aureus pathotypes in SouthWest, Nigeria. PLoS ONE. 2021; 16(2): e0247013" }, { "key": "ref13", "doi": "10.1128/jcm.36.12.3532-3539.1998", "unstructured": "Leski T, Oliveira D, Trzcinski K, Santos Sanchez I, Aires de Sousa M, Hryniewicz W, de Lencastre H. Clonal distribution of methicillinresistant Staphylococcus aureus in Poland. Journal of Clinical Microbiology.1998; 36:3532-3539" }, { "key": "ref14", "doi": "10.4314/ajcem.v5i2.7376", "unstructured": "Taiwo SS, Onile BA, Akanbi AA. Methicillin- resistant Staphylococcus aureus (MRSA) isolates in Ilorin, Nigeria. African Journal of Clinical and Experimental Microbiology.2004; 5: 189-197. [15].Shittu A, Lin J, Kolawole, D. Antimicrobial susceptibility patterns of Staphylococcus aureus and characterization of MRSA in Southwestern Nigeria. BMC Infectious Diseases.2006; 6:125" }, { "key": "ref15", "unstructured": "Alli Terry OA, Ogbolu DA, Akorede E, Onemu OM, Okanlawon BM. Distribution of mecA gene amongst Staphylococcus aureus isolates from Southwestern Nigeria. African Journal of Biomedical Research.2011; 14:9-16" }, { "key": "ref16", "doi": "10.1128/jcm.42.11.4947-4955.2004", "unstructured": "Zhang K, Sparling J, Chow B L, Elsayed S, Hussain Z, Church D L, Gregson DB, Louie T, Conly J. M. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative Staphylococci. Journal of Clinical Microbiology. 2004; 42:4947-4955" }, { "key": "ref17", "unstructured": "Harlow ED, Lane D. Antibodies: Laboratory manual.CSHL press. 1988; Pp 484–493" }, { "key": "ref18", "doi": "10.1203/pdr.0b013e31819dc44d", "unstructured": "Liu GY. Molecular pathogenesis of Staphylococcus aureus infection. Pediatric Research. 2009; 65:71R-77R" }, { "key": "ref19", "doi": "10.9734/jamb/2017/33655", "unstructured": "Fagade OE, Ezeamagu CO, Oyelade AA, Ogunjobi AA. Comparative study of antibiotic resistance of Staphylococcus species isolated from clinical and environmental samples. Assumption University Journal of Technology. 2010; 13: 165-169. List of Reviewer Professor Christopher Anyamene Department of Applied Microbiology and Brewing Nnamdi Azikiwe University, Awka Anambra State, Nigeria c.anyamene@unizik.edu.ng" } ], "record": { "registrant": "Longman Publishers", "registered": "2024-02-16", "updated": "2026-09-17", "issue_number": 1, "source": "crossref-api", "source_agency": "Crossref (member 25296)" } }, "url_after": "https://www.jst.org.in/index.php/pub/article/view/332", "sha256_before": null, "sha256_after": "84c712fb46093a3e77b42a25a5c76a0ee7b8c5c0940b1aa8a45f5bf1c8a595bc" }, { "seq": 2, "kind": "update", "at": "2026-09-29 23:59:41", "by": "Administrator (admin)", "by_kind": "user", "note": "record re-read from api.crossref.org", "changes": [ { "path": "agents.0.name.family", "from": " Celestina Chibuzo Ugwu", "to": "Celestina Chibuzo Ugwu" }, { "path": "agents.0.name.given", "from": " Celestina Chibuzo Ugwu", "to": "Celestina Chibuzo Ugwu" }, { "path": "container.titles.0.value", "from": "Journal of Science & Technology", "to": "Journal of Science & Technology" } ], "record_after": { "format": "smartscholars-doi-metadata/1.0", "doi": "10.46243/jst.2022.v7.i01.pp49-61", "referent": "Creation", "type": "JournalArticle", "structural_type": "Digital", "modes": [ "Visual" ], "characters": [ "Language" ], "titles": [ { "value": "Clonality of Methicillin-Resistant Staphylococcus aureus (MRSA) in Hospital and Community Samples in Nsukka, Nigeria.", "type": "PrincipalTitle", "lang": "en" } ], "identifiers": [ { "type": "DOI", "value": "10.46243/jst.2022.v7.i01.pp49-61" } ], "agents": [ { "role": "author", "name": { "given": "Celestina Chibuzo Ugwu", "family": "Celestina Chibuzo Ugwu" }, "sequence": "first", "identifiers": [ { "type": "ORCID", "value": "0000-0002-7685-9941", "verified": false } ] }, { "role": "publisher", "name": { "org": "Longman Publishers" } } ], "dates": { "published": "2023-07-26", "date_type": "PublicationDate", "online": "2023-07-26" }, "language": "en", "container": { "type": "Journal", "titles": [ { "value": "Journal of Science & Technology", "type": "PrincipalTitle" } ], "identifiers": [ { "type": "ISSN", "value": "2456-5660", "medium": "electronic" } ], "volume": "7", "issue": "1", "pages": { "first": "49", "last": "61" } }, "links": [ { "url": "https://www.jst.org.in/index.php/pub/article/view/332", "return_type": "text/html", "primary": true }, { "url": "https://www.jst.org.in/index.php/pub/article/download/332/296", "purpose": "text-mining", "return_type": "application/pdf" }, { "url": "https://www.jst.org.in/index.php/pub/article/download/332/2282", "purpose": "text-mining", "return_type": "application/xml" } ], "abstract": { "value": "Methicillin-resistant Staphylococcus aureus (MRSA) is a human pathogen that has emerged as a serious public health problem both in hospital and community settings. This study was aimed to determine the clonal variations of MRSA from healthcare and community environments. S. aureus was isolated and identified from hospital and community samples using standard laboratory procedures and MRSA strains were identified using Kirby Bauer disk diffusion method. Out of 505 S. aureus isolates recovered in this study, 295 (58.4%) were oxacillin resistant and were therefore considered to be methicillin-resistant Staphylococcus aureus (MRSA) strains. The prevalence of MRSA in the hospital environment was 34.8% and 28.7% in the community environment. Out of 21 selected MRSA isolates examined for mecA gene by PCR, only six (53, 69, 74, 84, 88 and 89) showed positive amplification for mecA. Of these six, one was a hospital isolate, while the other five were community isolates. Polymerase chain reaction (PCR) was used to detect both the Staphylococcus specific 16s rRNA and mecA genes with amplicon sizes of 750 bp and 310 bp, All oxacillin resistant isolates possessed the S. aureus specific sequence gene, but not all possessed the mecA gene. Genetic relatedness of the isolates was determined using protein profile from SDS-PAGE and restriction patterns from restriction fragment length polymorphism (RFLP). The protein profiles and restriction patterns revealed heterogeneity among the isolates. Phylogenetic analyses were done on the protein profiles and restriction patterns using bioinformatics software (UPGMA). The results revealed four different patterns suggesting that there are about four MRSA clones among the samples as well as genetic relatedness between hospital and community isolates.", "lang": "en" }, "license": { "url": "https://creativecommons.org/licenses/by/4.0", "start": "2023-07-26", "applies_to": "vor" }, "references": [ { "key": "ref1", "unstructured": "Chatterjee SS, Ray P, Aggarwal A, Das A, Sharma M. A community-based study on nasal carriage of Staphylococcus aureus. Indian Journal of Medical Research. 2009; 130: 742-748" }, { "key": "ref2", "doi": "10.1038/srep01902", "unstructured": "Prosperi M, Veras N, Azarian T, Rathore M.,Nolan D, Rand K, Cook RL, Johnson J, Morris JG, Salemi M. Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in the genomic era: a cross-sectional study. Scientic Reports3.2013" }, { "key": "ref3", "doi": "10.1016/j.ajic.2007.10.009", "unstructured": "Jarvis WR, Schlosser J, Chinn RY, Tweeten S, Jackson M. 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List of Reviewer Professor Christopher Anyamene Department of Applied Microbiology and Brewing Nnamdi Azikiwe University, Awka Anambra State, Nigeria c.anyamene@unizik.edu.ng" } ], "record": { "registrant": "Longman Publishers", "registered": "2024-02-16", "updated": "2026-09-17", "issue_number": 1, "source": "crossref-api", "source_agency": "Crossref (member 25296)" } }, "url_after": "https://www.jst.org.in/index.php/pub/article/view/332", "sha256_before": "84c712fb46093a3e77b42a25a5c76a0ee7b8c5c0940b1aa8a45f5bf1c8a595bc", "sha256_after": "297d8a591143b21dd463b6ccaabaf1dab532d4327d43d6656b3c9a2706014282" } ] }